Difference between revisions of "Part:BBa K1065204"

Revision as of 13:15, 3 October 2013

Efe+Bba_B0015 in BBa_K823024 (pXyl)

2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) from Pseudomonas Siringae pv, that catalyzes Ethylene biosynthesis from 2-oxoglutarate. The enzyme was inserted in the xylose inducible vector pSBBs4S-Pxyl constructed by the LMU Munich iGEM team 2012 for expression in Bacillus subtilis. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki. If interested in this part please contact us.

NOTE: We used a modified WORKING version of the pSpac vector that has been sent to us from the LMU Munich 2012 team.

Toxicity and sulfur test

We measured the optical density of cells induced and non induced:

Cells were induced with 1% of xylose. The graphic shows that the induced samples (blue trace) grow slightly slower than the controls (red trace).

Taking this measures we noticed that the induced colture had a really bad and not normal smell of sulfur and methane so we performed the sulfur test with the lead acetate strips:

The induced sample has darkened much more than the not induced, that's a strange result.

Usage and Biology

Sequence and Features