Difference between revisions of "Part:BBa K1149051:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
added different promoter RBS to increase expression
+
 
 +
HYbrid promoter construct includes J23104 + B0034 + native promoter and RBS, which precede phaCAB operon.
  
 
<h5> Partial sequence of the hybrid phaCAB construct ([https://parts.igem.org/Part:BBa_K1149051 BBa_K1149051]): </h5>
 
<h5> Partial sequence of the hybrid phaCAB construct ([https://parts.igem.org/Part:BBa_K1149051 BBa_K1149051]): </h5>

Latest revision as of 12:30, 3 October 2013


Hybrid promoter phaCAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 979
    Illegal BglII site found at 1804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 285
    Illegal NgoMIV site found at 356
    Illegal NgoMIV site found at 956
    Illegal NgoMIV site found at 1268
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 2199
    Illegal NgoMIV site found at 2221
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4065


Design Notes

HYbrid promoter construct includes J23104 + B0034 + native promoter and RBS, which precede phaCAB operon.

Partial sequence of the hybrid phaCAB construct (BBa_K1149051):

Hybrid_phaCAB.jpg

Source

comes from exisiting biobrick parts

References