Difference between revisions of "Part:BBa K1022112"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1022112 short</partinfo>
 
<partinfo>BBa_K1022112 short</partinfo>
Line 11: Line 10:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1022112 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1022112 SequenceAndFeatures</partinfo>
 +
 +
===Characterization===
 +
'''For more info, visit [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization  TU Delft iGEM13 Wiki]'''
 +
 +
'''Peptide Characterization:'''
 +
 +
An important part of the project TU Delft iGEM13 is inhibition of growth or killing of bacteria with the use of antimicrobial peptides (AMPs). In order to get an idea of the toxicity of our peptides we conducted several minimal inhibiting concentration (MIC) experiments. These MIC measurements where done on ''E. coli'', ''B. subtilis'' and ''S. delphini'', with the first as representative of our Gram-negative expression host, the second for the Gram-positive targets and the last for our specific target.
 +
 +
'''MIC determination:'''
 +
 +
The MIC of Magainin II on ''S. delphini, B. subtilis and E. coli'' were done according to the protocol described [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization here].
 +
Magainin II did not give a measurable reduction in growth below 40µM (Figure 1), making us decide not to proceed testing, as modeling showed it was not possible to reach these concentrations through expression in ''E.coli''.
 +
 +
 +
[[Image:Figure2 magainin TUD.jpg|600px|center]]
 +
 +
Figure 1: MIC of Magainin II on ''S. delphini''
 +
 +
 +
'''For Other peptides and their characterization please visit [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization Peptide characterization] on TU Delft iGEM13 Wiki'''
 +
 +
==Peptide Production==
 +
 +
'''For more info about the production of the peptide Magainin II, visit [http://2013.igem.org/Team:TU-Delft/Peptides#SUMO  Peptide production] on TU Delft iGEM13 Wiki.
 +
 +
Also check the construct used for the peptide production [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022103  BBa_K1022103] and more characterization.''
 +
  
  

Revision as of 12:17, 3 October 2013

PT7: RBS: Magainin II: His 6

This biobrick codes for the promoter pT7, Magainin II peptide with a His 6 tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization TU Delft iGEM13 Wiki]

Peptide Characterization:

An important part of the project TU Delft iGEM13 is inhibition of growth or killing of bacteria with the use of antimicrobial peptides (AMPs). In order to get an idea of the toxicity of our peptides we conducted several minimal inhibiting concentration (MIC) experiments. These MIC measurements where done on E. coli, B. subtilis and S. delphini, with the first as representative of our Gram-negative expression host, the second for the Gram-positive targets and the last for our specific target.

MIC determination:

The MIC of Magainin II on S. delphini, B. subtilis and E. coli were done according to the protocol described [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization here]. Magainin II did not give a measurable reduction in growth below 40µM (Figure 1), making us decide not to proceed testing, as modeling showed it was not possible to reach these concentrations through expression in E.coli.


Figure2 magainin TUD.jpg

Figure 1: MIC of Magainin II on S. delphini


For Other peptides and their characterization please visit [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization Peptide characterization] on TU Delft iGEM13 Wiki

Peptide Production

For more info about the production of the peptide Magainin II, visit [http://2013.igem.org/Team:TU-Delft/Peptides#SUMO Peptide production] on TU Delft iGEM13 Wiki.

Also check the construct used for the peptide production BBa_K1022103 and more characterization.