Difference between revisions of "Part:BBa K1104206:Experience"
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This part: AhpCpD1 is one of the improvement versions of BioBrick Part: ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCpD1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae'']. | This part: AhpCpD1 is one of the improvement versions of BioBrick Part: ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCpD1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae'']. | ||
| − | [[File: NYMU_46.png|frame|center|''' | + | [[File: NYMU_46.png|frame|center|'''AhpCpD1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104246 Part:BBa_K1104246])''']] |
We use the device: AhpCpD1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104246 Part:BBa_K1104246]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCp1000 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP. | We use the device: AhpCpD1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104246 Part:BBa_K1104246]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCp1000 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP. | ||
Revision as of 11:48, 3 October 2013
This part: AhpCpD1 is one of the improvement versions of BioBrick Part: ahpC promoter (Part:K362001) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCpD1 (Part:BBa_K1104204) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].
We use the device: AhpCpD1 +E0840 (Part:BBa_K1104246) includes promoter (Part:BBa_K1104204) and Part:BBa_E0840, induce the promoter by different concentrations of H2O2, and identify the strength. After AhpCp1000 (Part:BBa_K1104204) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
We tested the improved promoter: AhpCpD1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.
Results
Here is our result of AhpCpD1 testing:
Discussion
Applications of BBa_K1104206
User Reviews
UNIQf978ace82a5d3726-partinfo-00000000-QINU UNIQf978ace82a5d3726-partinfo-00000001-QINU