Difference between revisions of "Part:BBa K1065106:Design"

(→‎Design Notes)
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===References===
 
===References===
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1. Chang-Kui Ding, Chien Yi Wang (2003). The dual effects of methyl salicylate on ripening and expression of ethylene biosynthetic genes in tomato fruit, Plant Science Volume 164, Pages 589–596.

Revision as of 11:39, 3 October 2013


Wintergreen device with SAM: Plac + PCHBA + araCpBAD+ BSMT1 + Plac + SAM synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3107
    Illegal BamHI site found at 3856
    Illegal BamHI site found at 4736
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 793
    Illegal NgoMIV site found at 918
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1210
    Illegal NgoMIV site found at 1678
    Illegal AgeI site found at 2942
    Illegal AgeI site found at 5316
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3397
    Illegal SapI site found at 2924


Design Notes

The part was created subcloning BBa_K1065105 inside BBa_K1065102 following the standard assembly protocol.

Source

PchBA were extracted from Pseudomonas aeruginosa.
AraC-pBAD was extracted from E. coli.
BSMT1 was extracted from Petunia x hybrida.
SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.

References

1. Chang-Kui Ding, Chien Yi Wang (2003). The dual effects of methyl salicylate on ripening and expression of ethylene biosynthetic genes in tomato fruit, Plant Science Volume 164, Pages 589–596.