Difference between revisions of "Part:BBa K1022111"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1022111 short</partinfo>
 
<partinfo>BBa_K1022111 short</partinfo>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1022111 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1022111 SequenceAndFeatures</partinfo>
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===Characterization===
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'''For more info, visit [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization  TU Delft iGEM13 Wiki]'''
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'''Peptide Characterization:'''
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An important part of the project is inhibition of growth or killing of bacteria with the use of antimicrobial peptides (AMPs). In order to get an idea of the toxicity of our peptides we conducted several minimal inhibiting concentration (MIC) experiments. These MIC measurements where done on ''E. coli'', ''B. subtilis'' and ''S. delphini'', with the first as representative of our Gram-negative expression host, the second for the Gram-positive targets and the last for our specific target.
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'''MIC determination:'''
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The MIC of signiferin on ''S. delphini, B. subtilis and E. coli'' were done according to the protocol described [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization here]. ''S. delphini'' is the most sensitive for signiferin, as the MIC was determined to be 1µM (Figure 1A), no growth is seen at this concentration. The MIC for ''B. subtilis'' was determined to be 10µM (Figure 1B).
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[[Image:Figure1 TUD.jpg|450px|center]]
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Revision as of 11:18, 3 October 2013

PT7: RBS: Signiferin: His 6

This Biobrick codes for promoter pT7 followed by Signiferin peptide with a His 6 tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization TU Delft iGEM13 Wiki]

Peptide Characterization:

An important part of the project is inhibition of growth or killing of bacteria with the use of antimicrobial peptides (AMPs). In order to get an idea of the toxicity of our peptides we conducted several minimal inhibiting concentration (MIC) experiments. These MIC measurements where done on E. coli, B. subtilis and S. delphini, with the first as representative of our Gram-negative expression host, the second for the Gram-positive targets and the last for our specific target.

MIC determination:

The MIC of signiferin on S. delphini, B. subtilis and E. coli were done according to the protocol described [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization here]. S. delphini is the most sensitive for signiferin, as the MIC was determined to be 1µM (Figure 1A), no growth is seen at this concentration. The MIC for B. subtilis was determined to be 10µM (Figure 1B).


Figure1 TUD.jpg