Difference between revisions of "Part:BBa K1022114"
Line 24: Line 24:
 
{| class="wikitable"
 
{| class="wikitable"
 
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! No cells + 1mM !!0.1mM !0.2m !!0.3mM !! 0.4mM!! 0.5mM !!0.6mM !! 0.7mM!! 0.8mM !! 0.9mM !! 1mM Cell + No IPTG
+
!      !! No cells + 1mM !!0.1mM !!0.2m !!0.3mM !! 0.4mM!! 0.5mM !!0.6mM !! 0.7mM!! 0.8mM !! 0.9mM !! 1mM Cell + No IPTG
 
|-
 
|-
 
| LB(µL) || 90 || 94 ||93||92 ||91 ||90 ||89 ||88 ||87 ||86 ||85 ||95  
 
| LB(µL) || 90 || 94 ||93||92 ||91 ||90 ||89 ||88 ||87 ||86 ||85 ||95  
 
|-
 
|-
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| Example || Example || Example
+
| Cells(µL) || - || 5 || 5 || 5|| 5|| 5|| 5|| 5|| 5|| 5|| 5 || 5
 
|-
 
|-
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| Example || Example || Example
+
| 10X IPTG(µL) || 10 || 1 || 2|| 3|| 4|| 5|| 6|| 7|| 8||9|| 10 || -
 
|}
 
|}
  
−
 
−
No cells + 1mM 0.1mM 0.2m 0.3mM 0.4mM 0.5mM 0.6mM 0.7mM 0.8mM 0.9mM 1mM Cell + No IPTG
 
−
LB(µL) 90 94 93 92 91 90 89 88 87 86 85 95
 
−
Cells(µL) - 5 5 5 5 5 5 5 5 5 5 5
 
−
10X IPTG(µL) 10 1 2 3 4 5 6 7 8 9 10 -
 
  
  

Revision as of 10:05, 3 October 2013

pT7 : Lysis Device

This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1404
    Illegal NheI site found at 1427
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1009
    Illegal AgeI site found at 1079
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1660


Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]

The construct is made by ligating the pT7 promoter BBa_I712074 in front of the lysis device from the biobrick BBa_K112808. This is analyze how the lysis device can be controlled if needed.

114.jpg

Description : The E.coli cells transformed with pT7 lysis cassette BBa_K1022114 is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick BBa_K1022114. At time point, 160 minutes, IPTG is added according to the table below.

No cells + 1mM 0.1mM 0.2m 0.3mM 0.4mM 0.5mM 0.6mM 0.7mM 0.8mM 0.9mM 1mM Cell + No IPTG
LB(µL) 90 94 93 92 91 90 89 88 87 86 85 95
Cells(µL) - 5 5 5 5 5 5 5 5 5 5 5
10X IPTG(µL) 10 1 2 3 4 5 6 7 8 9 10 -


Lysis TU Delft.jpg