Difference between revisions of "Part:BBa K1022114"

Revision as of 10:01, 3 October 2013

pT7 : Lysis Device

This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1404
Illegal NheI site found at 1427
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1009
Illegal AgeI site found at 1079
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1660

Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]

The construct is made by ligating the pT7 promoter BBa_I712074 in front of the lysis device from the biobrick BBa_K112808. This is analyze how the lysis device can be controlled if needed.

Description : The E.coli cells transformed with pT7 lysis cassette BBa_K1022114 is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick BBa_K1022114. At time point, 160 minutes, IPTG is added according to the table below.

No cells + 1mM	0.1mM !0.2m	0.3mM	0.4mM	0.5mM	0.6mM	0.7mM	0.8mM	0.9mM	1mM Cell + No IPTG
LB(µL)	90	94	93	92	91	90	89	88	87	86	85	95
Example	Example	Example
Example	Example	Example

	No cells + 1mM 	0.1mM 	0.2m 	0.3mM 	0.4mM 	0.5mM 	0.6mM 	0.7mM 	0.8mM 	0.9mM 	1mM 	Cell + No IPTG

LB(µL) 90 94 93 92 91 90 89 88 87 86 85 95 Cells(µL) - 5 5 5 5 5 5 5 5 5 5 5 10X IPTG(µL) 10 1 2 3 4 5 6 7 8 9 10 -

@@ Line 23: / Line 23: @@
 {| class="wikitable"
-|- No cells + 1mM 0.1mM 0.2m 0.3mM 0.4mM 0.5mM 	0.6mM 0.7mM 0.8mM 0.9mM 1mM Cell + No IPTG
 |-
-| LB(µL) 	|| 90 	|| 94 ||93 	 ||92 	 ||91 	 ||90 	 ||89 ||88 	||87 	 ||86  ||	85 ||	95 ||
+! No cells + 1mM !!0.1mM !0.2m !!0.3mM !! 0.4mM!! 0.5mM !!0.6mM !! 0.7mM!! 0.8mM !! 0.9mM !! 1mM Cell + No IPTG
+|-
+| LB(µL) || 90 	|| 94 ||93||92 	||91 ||90 ||89 ||88 ||87 ||86 ||85 ||95
 |-
 | Example || Example || Example