Difference between revisions of "Part:BBa K1022114"
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Description : | Description : | ||
The <i>E.coli</i> cells transformed with pT7 lysis cassette [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114 BBa_K1022114] is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114 BBa_K1022114]. At time point, 160 minutes, IPTG is added according to the table below. | The <i>E.coli</i> cells transformed with pT7 lysis cassette [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114 BBa_K1022114] is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114 BBa_K1022114]. At time point, 160 minutes, IPTG is added according to the table below. | ||
+ | |||
+ | {| class="wikitable" | ||
+ | |- No cells + 1mM 0.1mM 0.2m 0.3mM 0.4mM 0.5mM 0.6mM 0.7mM 0.8mM 0.9mM 1mM Cell + No IPTG | ||
+ | |- | ||
+ | | LB(µL) || 90 || 94 ||93 ||92 ||91 ||90 ||89 ||88 ||87 ||86 || 85 || 95 || | ||
+ | |- | ||
+ | | Example || Example || Example | ||
+ | |- | ||
+ | | Example || Example || Example | ||
+ | |} | ||
+ | |||
No cells + 1mM 0.1mM 0.2m 0.3mM 0.4mM 0.5mM 0.6mM 0.7mM 0.8mM 0.9mM 1mM Cell + No IPTG | No cells + 1mM 0.1mM 0.2m 0.3mM 0.4mM 0.5mM 0.6mM 0.7mM 0.8mM 0.9mM 1mM Cell + No IPTG |
Revision as of 09:59, 3 October 2013
pT7 : Lysis Device
This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1404
Illegal NheI site found at 1427 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1009
Illegal AgeI site found at 1079 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1660
Characterization
For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]
The construct is made by ligating the pT7 promoter BBa_I712074 in front of the lysis device from the biobrick BBa_K112808. This is analyze how the lysis device can be controlled if needed.
Description : The E.coli cells transformed with pT7 lysis cassette BBa_K1022114 is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick BBa_K1022114. At time point, 160 minutes, IPTG is added according to the table below.
LB(µL) | 90 | 94 | 93 | 92 | 91 | 90 | 89 | 88 | 87 | 86 | 85 | 95 | |
Example | Example | Example | |||||||||||
Example | Example | Example |
No cells + 1mM 0.1mM 0.2m 0.3mM 0.4mM 0.5mM 0.6mM 0.7mM 0.8mM 0.9mM 1mM Cell + No IPTG
LB(µL) 90 94 93 92 91 90 89 88 87 86 85 95 Cells(µL) - 5 5 5 5 5 5 5 5 5 5 5 10X IPTG(µL) 10 1 2 3 4 5 6 7 8 9 10 -