Difference between revisions of "Part:BBa K1022114"
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The <i>E.coli</i> cells transformed with pT7 lysis cassette [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114  BBa_K1022114] is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114  BBa_K1022114].  At time point, 160 minutes, IPTG is added according to the table below.  
 
The <i>E.coli</i> cells transformed with pT7 lysis cassette [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114  BBa_K1022114] is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114  BBa_K1022114].  At time point, 160 minutes, IPTG is added according to the table below.  
  
−
                <table >
+
No cells + 1mM 0.1mM 0.2m 0.3mM 0.4mM 0.5mM 0.6mM 0.7mM 0.8mM 0.9mM 1mM Cell + No IPTG
−
                    <tr>
+
LB(µL) 90 94 93 92 91 90 89 88 87 86 85 95
−
                        <td></td>
+
Cells(µL) - 5 5 5 5 5 5 5 5 5 5 5
−
                        <td>
+
10X IPTG(µL) 10 1 2 3 4 5 6 7 8 9 10 -
−
                        No cells + 1mM
+
−
                        </td>
+
−
                        <td >0.1mM</td>
+
−
                        <td>0.2m</td>
+
−
                        <td>0.3mM</td>
+
−
                        <td>0.4mM</td>
+
−
                        <td>0.5mM</td>
+
−
                        <td>0.6mM</td>
+
−
                        <td>
+
−
                          0.7mM
+
−
                        </td>
+
−
                        <td>
+
−
                          0.8mM
+
−
                        </td>
+
−
                        <td>
+
−
                          0.9mM
+
−
                        </td>
+
−
                        <td>
+
−
                          1mM
+
−
                        </td>
+
−
                        <td>
+
−
                          Cell + No IPTG
+
−
                        </td>
+
−
                    </tr>
+
−
 
+
−
    <tr>
+
−
                        <td>
+
−
                        LB(µL)
+
−
                        </td>
+
−
                        <td >
+
−
                          90
+
−
                        </td>
+
−
                        <td>
+
−
                            94
+
−
                        </td>
+
−
                        <td>93</td>
+
−
                        <td>92</td>
+
−
                                              <td>91</td>
+
−
                        <td>90</td>
+
−
                        <td>89</td>
+
−
                        <td>88</td>
+
−
                        <td>87</td>
+
−
                        <td>86</td>
+
−
                        <td>85</td>
+
−
                        <td>95</td>     
+
−
                    </tr>
+
−
 
+
−
<tr>
+
−
                        <td>
+
−
                        Cells(µL)
+
−
                        </td>
+
−
                        <td>-</td>   
+
−
                        <td>5</td>
+
−
                        <td>5</td>     
+
−
                        <td>5</td>
+
−
                        <td>5</td>
+
−
                        <td>5</td> 
+
−
                        <td>5</td> 
+
−
                        <td>5</td>   
+
−
                        <td>5</td>       
+
−
                        <td>5</td> 
+
−
                        <td>5</td>
+
−
                        <td>5</td>               
+
−
                     
+
−
                    </tr>
+
−
 
+
−
<tr>
+
−
                        <td>
+
−
                        10X IPTG(µL)
+
−
                        </td>
+
−
                        <td>10</td>   
+
−
                        <td>1</td>
+
−
                        <td>2</td>     
+
−
                        <td>3</td>
+
−
                        <td>4</td> 
+
−
                        <td>5</td>
+
−
                        <td>6</td>
+
−
                        <td>7</td>
+
−
                        <td>8</td>
+
−
                        <td>9</td> 
+
−
                        <td>10</td>     
+
−
                        <td>-</td>               
+
−
                     
+
−
                    </tr>
+
−
 
+
−
</table>
+
−
 
+
−
 
+
  
  

Revision as of 09:52, 3 October 2013

pT7 : Lysis Device

This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1404
    Illegal NheI site found at 1427
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1009
    Illegal AgeI site found at 1079
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1660


Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]

The construct is made by ligating the pT7 promoter BBa_I712074 in front of the lysis device from the biobrick BBa_K112808. This is analyze how the lysis device can be controlled if needed.

114.jpg

Description : The E.coli cells transformed with pT7 lysis cassette BBa_K1022114 is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick BBa_K1022114. At time point, 160 minutes, IPTG is added according to the table below. 

	No cells + 1mM 	0.1mM 	0.2m 	0.3mM 	0.4mM 	0.5mM 	0.6mM 	0.7mM 	0.8mM 	0.9mM 	1mM 	Cell + No IPTG

LB(µL) 90 94 93 92 91 90 89 88 87 86 85 95 Cells(µL) - 5 5 5 5 5 5 5 5 5 5 5 10X IPTG(µL) 10 1 2 3 4 5 6 7 8 9 10 -


Lysis TU Delft.jpg