Difference between revisions of "Part:BBa K1022114"
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'''For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]''' | '''For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]''' | ||
− | The construct is made by ligating the pT7 promoter | + | The construct is made by ligating the pT7 promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074 !!]in front of the lysis device from the biobrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112808" target="_blank"> BBa_K112808</a>. This is analyze how the lysis device can be controlled if needed. |
<center> | <center> |
Revision as of 08:17, 3 October 2013
pT7 : Lysis Device
This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1404
Illegal NheI site found at 1427 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1009
Illegal AgeI site found at 1079 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1660
Characterization
For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]
The construct is made by ligating the pT7 promoter BBa_I712074 !!in front of the lysis device from the biobrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112808" target="_blank"> BBa_K112808</a>. This is analyze how the lysis device can be controlled if needed.
<img src=""/>
Figure 2: Part BBa_K1022114
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