Difference between revisions of "Part:BBa K1022114"
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<partinfo>BBa_K1022114 short</partinfo>
 
<partinfo>BBa_K1022114 short</partinfo>
  
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This bio-brick codes for the promoter pT7 followed by the lysis device.
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This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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===Characterisation===
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===Characterization===
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'''For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch!!]'''
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The construct is made by ligating the pT7 promoter(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074" target="_blank">BBa_I712074</a>) in front of the lysis device from the biobrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112808" target="_blank"> BBa_K112808</a>. This is analyze how the lysis device can be controlled if needed.
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<center>
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<a href="https://static.igem.org/mediawiki/2013/c/c0/114.jpg" target="_blank"><img src="https://static.igem.org/mediawiki/2013/c/c0/114.jpg"/></a>
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</center>
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<center>
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<p>Figure 2: Part BBa_K1022114</p></div></center>
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<br>
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Revision as of 08:14, 3 October 2013

pT7 : Lysis Device

This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1404
    Illegal NheI site found at 1427
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1009
    Illegal AgeI site found at 1079
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1660


Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch!!]

The construct is made by ligating the pT7 promoter(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074" target="_blank">BBa_I712074</a>) in front of the lysis device from the biobrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112808" target="_blank"> BBa_K112808</a>. This is analyze how the lysis device can be controlled if needed.

<a href="114.jpg" target="_blank"><img src="114.jpg"/></a>

Figure 2: Part BBa_K1022114

</div>