Difference between revisions of "Part:BBa K1051133"

 
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<partinfo>BBa_K1051133 parameters</partinfo>
 
<partinfo>BBa_K1051133 parameters</partinfo>
 
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===principle===
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<p>Histones are nuclear proteins package DNA into nucleosomes, and they are responsible for maintaining the shape and structure of a nucleosome. One chromatin molecule is composed of at least one of each core histones per 100 base pairs of DNA.[The Nucleosome: From Genomic Organization to Genomic Regulation.] There are five families of histones known to date, termed H1/H5, H2A, H2B, H3, and H4. H2A is considered a core histone, along with H2B, H3 and H4. Core formation first occurs through the interaction of two H2A molecules(Acid, 2004). Then, H2A forms a dimer with H2B; the core molecule is complete when H3-H4 also attaches to form a tetramer.</p>
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===Results===
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https://static.igem.org/mediawiki/2013/b/ba/Nucleus.jpg
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===Reference===
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Acid, S. A. (2004). Lehninger principles of biochemistry

Revision as of 07:22, 3 October 2013

K900002+GFP

K900002(N terminal)+GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 97
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1058


principle

Histones are nuclear proteins package DNA into nucleosomes, and they are responsible for maintaining the shape and structure of a nucleosome. One chromatin molecule is composed of at least one of each core histones per 100 base pairs of DNA.[The Nucleosome: From Genomic Organization to Genomic Regulation.] There are five families of histones known to date, termed H1/H5, H2A, H2B, H3, and H4. H2A is considered a core histone, along with H2B, H3 and H4. Core formation first occurs through the interaction of two H2A molecules(Acid, 2004). Then, H2A forms a dimer with H2B; the core molecule is complete when H3-H4 also attaches to form a tetramer.

Results

Nucleus.jpg

Reference

Acid, S. A. (2004). Lehninger principles of biochemistry