Difference between revisions of "Part:BBa K1197004"
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<partinfo>BBa_K1197004 short</partinfo> | <partinfo>BBa_K1197004 short</partinfo> | ||
This is a LacI producing part which is proper for B.subtilis. Any promoter can be added to the downstream of that part to obtain a LacI coding device. | This is a LacI producing part which is proper for B.subtilis. Any promoter can be added to the downstream of that part to obtain a LacI coding device. | ||
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| + | This part is obtained from BBa_I732820 part by changing the original RBS sequence with RBS SpoVG via specific primers. And LacI was codon optimized for expression in B.subtilis. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 03:36, 3 October 2013
RBS SpoVG + LacI + DT
This is a LacI producing part which is proper for B.subtilis. Any promoter can be added to the downstream of that part to obtain a LacI coding device.
This part is obtained from BBa_I732820 part by changing the original RBS sequence with RBS SpoVG via specific primers. And LacI was codon optimized for expression in B.subtilis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]