Difference between revisions of "Part:BBa K1149010"
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<p>(image from BRENDA)</p>
 
<p>(image from BRENDA)</p>
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<h2>Characterisation</h2>
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Our Phaz1 construct was tested for induction by addition of Xylose.
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https://static.igem.org/mediawiki/2013/thumb/d/d2/Phaz1.png/450px-Phaz1.png
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'''Phaz1 growth assay.''' MG1655 ''E. coli'' were grown for 6h with 0% and 2% Xylose to induce Phaz1 BBa_K1149010. A two-tailed t-test, with p = 0.4181 shows that there is no significant difference between induction and no induction with regards to growth inhibition, as the value exceeds the critical value of 0.05. Growth was at 37°C with shaking. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.
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<b>Conclusion: There is no growth inhibition caused by induction in Phaz1. </b>
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<h2>References: </h2>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 23:16, 2 October 2013

Phaz1 expression

Depolymerises poly 3-hydroxybutyrate extracellularly Reaction_phaz.jpg

(image from BRENDA)

Characterisation

Our Phaz1 construct was tested for induction by addition of Xylose.

450px-Phaz1.png

Phaz1 growth assay. MG1655 E. coli were grown for 6h with 0% and 2% Xylose to induce Phaz1 BBa_K1149010. A two-tailed t-test, with p = 0.4181 shows that there is no significant difference between induction and no induction with regards to growth inhibition, as the value exceeds the critical value of 0.05. Growth was at 37°C with shaking. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.

Conclusion: There is no growth inhibition caused by induction in Phaz1.

References:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BglII site found at 981
    Illegal XhoI site found at 1261
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 301
    Illegal NgoMIV site found at 776
    Illegal NgoMIV site found at 827
    Illegal NgoMIV site found at 1021
    Illegal NgoMIV site found at 1229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 405
    Illegal SapI site found at 999