Difference between revisions of "Part:BBa K1151003:Design"
LeleBiotec (Talk | contribs) (→Design Notes) |
LeleBiotec (Talk | contribs) (→Design Notes) |
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This part was amplified by PCR from the plasmid received. | This part was amplified by PCR from the plasmid received. | ||
| − | [[File:pnikRUnisalento.jpg]] | + | [[File:pnikRUnisalento.jpg]] |
Figure 1: PCR results of pnikR. | Figure 1: PCR results of pnikR. | ||
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'''Primers used''' (including Biobricks' Prefix and Suffix, lowercase): | '''Primers used''' (including Biobricks' Prefix and Suffix, lowercase): | ||
| − | '' | + | ''pnikFor:'' gtttcttcgaattcgcggccgcttctagagAATTCAAACGCTCTTATG |
| − | '' | + | ''pnikRev:'' gtttcttcctgcagcggccgctactagtaTGAGAAAAATCCTTTTTTG |
===Source=== | ===Source=== | ||
Revision as of 19:25, 2 October 2013
pnikR
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was amplified by PCR from the plasmid received.
Figure 1: PCR results of pnikR.
Primers used (including Biobricks' Prefix and Suffix, lowercase):
pnikFor: gtttcttcgaattcgcggccgcttctagagAATTCAAACGCTCTTATG
pnikRev: gtttcttcctgcagcggccgctactagtaTGAGAAAAATCCTTTTTTG
Source
We received this part by Dr. Alberto Danielli, a researcher in molecular biology at the Department of Pharmacology and Biotechnology at the University of Bologna. He sent us the double divergent promoter (from the genome of H. pylori G27) inserted into a pGEM T-Easy plasmid (as pNKTB).
References
1. Delany et al, In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in Helicobacter pylori. J Bacteriol. 2005 Nov;187(22):7703-15.