Difference between revisions of "Part:BBa K1111005"
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This plasmid contains the constitutive promoter BBa_J23119, that was designed by the 2007 Berkley Team. We inserted it into the biobrick BBa_I746908 instead of the AraC promoter. The cells that contained this construct turned bright green. We used them as a positive control to characterize two of our other parts which contain the same plasmid but have inducible pormoters instead. | This plasmid contains the constitutive promoter BBa_J23119, that was designed by the 2007 Berkley Team. We inserted it into the biobrick BBa_I746908 instead of the AraC promoter. The cells that contained this construct turned bright green. We used them as a positive control to characterize two of our other parts which contain the same plasmid but have inducible pormoters instead. | ||
Revision as of 19:12, 2 October 2013
Constitutive promoter inducing GFP expression
This plasmid contains the constitutive promoter BBa_J23119, that was designed by the 2007 Berkley Team. We inserted it into the biobrick BBa_I746908 instead of the AraC promoter. The cells that contained this construct turned bright green. We used them as a positive control to characterize two of our other parts which contain the same plasmid but have inducible pormoters instead.
Usage and Biology
This plasmid had many applications, expecially in our igem project: 1.) it is used as a positive control for any promoter that is to be tested 2.) We used it for our synthetic biology Kit that was part of our Human Practice. As the fluorescence is seen by the naked eye, it makes a very nice experiments to make students interested in synthetic biology. 3.) the plasmid itself confers a chloramphenicol to the bacteria.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 74