Difference between revisions of "Part:BBa K1111005"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1111005 short</partinfo>
 
<partinfo>BBa_K1111005 short</partinfo>
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This plasmid contains the constitutive promoter BBa_J23119, that was designed by the 2007 Berkley Team. We inserted it into the biobrick BBa_I746908 instead of the AraC promoter. The cells that contained this construct turned bright green. We used them as a positive control to characterize two of our other parts which contain the same plasmid but have inducible pormoters instead.
  
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This Plasmid should induce GFP expression on external acidification
 
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1111005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1111005 SequenceAndFeatures</partinfo>

Revision as of 19:09, 2 October 2013

Constitutive promoter inducing GFP expression This plasmid contains the constitutive promoter BBa_J23119, that was designed by the 2007 Berkley Team. We inserted it into the biobrick BBa_I746908 instead of the AraC promoter. The cells that contained this construct turned bright green. We used them as a positive control to characterize two of our other parts which contain the same plasmid but have inducible pormoters instead.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 74