Difference between revisions of "Part:BBa K1151002:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The double divergent promoter was inserted in a pGEM-T Easy vector.
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This part was amplified by PCR from the plasmid received.
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                                    [[File:intergenicaUnisalento.jpg]]
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'''Figure 1:''' PCR results of the intergenic region.
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Primers used (including Biobricks' Prefix and Suffix, lowercase):
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''IntergenicaFor:'' gtttcttcgaattcgcggccgcttctagagTGAGAAAAATCCTTTTTTG
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''pexbrev:'' gtttcttcctgcagcggccgctactagtaGCACCCTATAAGAAGGCATC
  
 
===Source===
 
===Source===

Revision as of 19:03, 2 October 2013

Double divergent promoter pnikR-pexbB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified by PCR from the plasmid received. 

                                    IntergenicaUnisalento.jpg

Figure 1: PCR results of the intergenic region.

Primers used (including Biobricks' Prefix and Suffix, lowercase):

IntergenicaFor: gtttcttcgaattcgcggccgcttctagagTGAGAAAAATCCTTTTTTG

pexbrev: gtttcttcctgcagcggccgctactagtaGCACCCTATAAGAAGGCATC

Source

This part comes from H. pylori genomic sequence and it was amplified by PCR.

References

1. Delany et al, In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in Helicobacter pylori. J Bacteriol. 2005 Nov;187(22):7703-15.