Difference between revisions of "Part:BBa K1111005:Experience"

Revision as of 18:34, 2 October 2013

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Applications of BBa_K1111005

We constructed this plasmid in order to use it as a positive control for promoters that are inducible and inserten in front of the same reporter gene (superfolded GFP from iGEM). As it is constitutively expressed, the fluorescence can be used at least as a qualitative comparison to promoters that are induced.

Gibson Assembly and Primers

We used Gibson Assembly to insert this constitutive promoter into the pSB1C3 Backbone. We used the biobrick BBa_I746908 where we remove the Arac promoter but left the superfolded GFP. This way, the latter was under control of our Cad promoter.

Figure 1: History of the Gibson Assembly including Primers

Sequencing

We sequenced both the Promoter and the superfolded GFP. Both sequencing results showed that there were no mutations in the sequences.

Figure 1: Sequencing result of the constitutive promoter using one foreward Primer that binds directly to the beginning of the promoter

Figure 1: Sequencing result of the superfolded GFP using one foreward Primer that binds to the VF2 bindning site in the pSB1C3 plasmid

User Reviews

UNIQc24237466fd0d8a3-partinfo-00000000-QINU UNIQc24237466fd0d8a3-partinfo-00000001-QINU