Difference between revisions of "Part:BBa K1151002:Design"

(Design Notes)
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<partinfo>BBa_K1151002 short</partinfo>
 
<partinfo>BBa_K1151002 short</partinfo>
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===Design Notes===
 
===Design Notes===
The double divergent promoter was inserted in pGEM-T Easy.
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The double divergent promoter was inserted in a pGEM-T Easy vector.
  
 
===Source===
 
===Source===

Revision as of 18:08, 2 October 2013

Double divergent promoter pnikR-pexbB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The double divergent promoter was inserted in a pGEM-T Easy vector.

Source

This part comes from H. pylori genomic sequence and it was amplified by PCR.

References

1. Delany et al, In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in Helicobacter pylori. J Bacteriol. 2005 Nov;187(22):7703-15.