Difference between revisions of "Part:BBa K1111002:Experience"
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===Gibson Assembly=== | ===Gibson Assembly=== | ||
| + | We used Gibson Assembly to insert the Cad promoter into the pSB1C3 Backbone. We used the biobrick BBa_I746908 where we remove the Arac promoter but left the superfolded GFP. This way, the latter was under control of our Cad promoter. | ||
===Sequencing=== | ===Sequencing=== | ||
Revision as of 17:45, 2 October 2013
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Applications of BBa_K1111002
We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.
Gibson Assembly
We used Gibson Assembly to insert the Cad promoter into the pSB1C3 Backbone. We used the biobrick BBa_I746908 where we remove the Arac promoter but left the superfolded GFP. This way, the latter was under control of our Cad promoter.
Sequencing
User Reviews
UNIQd9ee4567b4047581-partinfo-00000000-QINU UNIQd9ee4567b4047581-partinfo-00000001-QINU