Difference between revisions of "Part:BBa K1151002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The double divergent promoter was inserted | + | The double divergent promoter was inserted in pGEM-T Easy. |
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===Source=== | ===Source=== | ||
Revision as of 15:56, 2 October 2013
Double divergent promoter pnikR-pexbB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The double divergent promoter was inserted in pGEM-T Easy.
Source
This part comes from H. pylori genomic sequence and it was amplified by PCR.
References
1. Delany et al, In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in Helicobacter pylori. J Bacteriol. 2005 Nov;187(22):7703-15.