Difference between revisions of "Part:BBa K1150046"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Fusion of [https://parts.igem.org/Part:BBa_K1150045 RNAimer with bla-target 2 and VEGF-target 4] and [https://parts.igem.org/Part:BBa_K1150044 RNAimer with bla-target 3 and bla-target 4]. This RNAimer was build via iGEM-ligation method and contains the DNA-target sequences of three areas of the bla-gene and one area of the VEGF locus. This enables multiple gene targeting throught one plasmid. The transfection with such a multiple target-plasmid avoids additional cell stress compared to transfection with serveral single target-plasmids. | Fusion of [https://parts.igem.org/Part:BBa_K1150045 RNAimer with bla-target 2 and VEGF-target 4] and [https://parts.igem.org/Part:BBa_K1150044 RNAimer with bla-target 3 and bla-target 4]. This RNAimer was build via iGEM-ligation method and contains the DNA-target sequences of three areas of the bla-gene and one area of the VEGF locus. This enables multiple gene targeting throught one plasmid. The transfection with such a multiple target-plasmid avoids additional cell stress compared to transfection with serveral single target-plasmids. | ||
Revision as of 13:36, 2 October 2013
uniCAS RNaimer multiple targets to Bla target 2, 3, 4 and VEGF 4
| uniCAS RNaimer multiple targets to Bla target 2 and VEGF target 4 | |
|---|---|
| Function | fourfold targeting with one plasmid |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Usage and Biology
Fusion of RNAimer with bla-target 2 and VEGF-target 4 and RNAimer with bla-target 3 and bla-target 4. This RNAimer was build via iGEM-ligation method and contains the DNA-target sequences of three areas of the bla-gene and one area of the VEGF locus. This enables multiple gene targeting throught one plasmid. The transfection with such a multiple target-plasmid avoids additional cell stress compared to transfection with serveral single target-plasmids.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 224
Illegal BglII site found at 1061
Illegal BglII site found at 1898
Illegal BglII site found at 2735 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216
Illegal BsaI.rc site found at 1053
Illegal BsaI.rc site found at 1890
Illegal BsaI.rc site found at 2727