Difference between revisions of "Part:BBa K1150027"

 
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<partinfo>BBa_K1150027 short</partinfo>
 
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===Usage and Biology===
 
===Usage and Biology===
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Fusion protein of [https://parts.igem.org/Part:BBa_K1150004 PhyB] and [https://parts.igem.org/Part:BBa_K1150002 KRAB]. This fusion protein PhyB-KRAB is an interaction partner of [https://parts.igem.org/Part:BBa_K1150025 dCas9-PIF]. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. When the system is exposed to red light (660 nm), the Phytochrome B receptor binds to the Phytochrome interaction actor (PIF). So it recruits the dCas9-KRAB protein. The PhyB-PIF binding can be abolished by illumination with far-red light (740nm.) This system enables  repression of gene expression induced by red light.
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===References===
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Müller, K.; Engesser, R.; Metzger, S.; Schulz, S.; Kampf, M. M.; Busacker, M. et al. (Nucleid Acid Research, 2013): A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells.
  
 
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Revision as of 12:26, 2 October 2013

uniCAS Red Light Switch Part II - Repressor


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 1076
    Illegal BamHI site found at 1158
    Illegal XhoI site found at 1109
    Illegal XhoI site found at 1128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2803
    Illegal SapI site found at 1325
    Illegal SapI.rc site found at 2767