Difference between revisions of "Part:BBa K1151011"
LeleBiotec (Talk | contribs) (→PCR with VF2 and VR2 primers) |
LeleBiotec (Talk | contribs) (→PCR with VF2 and VR2 primers) |
||
| Line 12: | Line 12: | ||
===PCR with VF2 and VR2 primers=== | ===PCR with VF2 and VR2 primers=== | ||
| − | + | [[File:PCR77.jpg]] | |
'''Figure 2:''' PCR results. | '''Figure 2:''' PCR results. | ||
Revision as of 12:11, 2 October 2013
GFP nickel regulated 3
This is a nickel sensitive system driven by the nikR-exbB intergenic region (BBa_K1151002) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.
Boiling prep and digestion with restriction enzymes
Figure 1: Digestion result of boiling prep plasmids.
PCR with VF2 and VR2 primers
Figure 2: PCR results.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 919