Difference between revisions of "Part:BBa K1151011"

Revision as of 12:07, 2 October 2013

GFP nickel regulated 3

This is a nickel sensitive system driven by the nikR-exbB intergenic region (BBa_K1151002) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.

Boiling prep and digestion with restriction enzymes

Figure 1: Digestion result of boiling prep plasmids.

PCR with VF2 and VR2 primers

Figure 2: PCR results.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 919

@@ Line 4: / Line 4: @@
 This is a nickel sensitive system driven by the nikR-exbB intergenic region ([[BBa_K1151002]]) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR ([[BBa_K1151000]]) as trascription factor.
-<!-- Add more about the biology of this part here
+===Boiling prep and digestion with restriction enzymes===
-===Usage and Biology===
+[[File:EPEP3.jpg]]
+'''Figure 1:''' Digestion result of boiling prep plasmids.
+===PCR with VF2 and VR2 primers===
+[[File:PCR77.jpg]]
+'''Figure 2:''' PCR results.
 <!-- -->