Difference between revisions of "Part:BBa K1151010"

(PCR with VF2 and VR2 primers)
(Boiling prep and digestion with restriction enzymes)
Line 6: Line 6:
 
===Boiling prep and digestion with restriction enzymes===
 
===Boiling prep and digestion with restriction enzymes===
  
              [[File:EPEP1.jpg]]
+
                          [[File:EPEP1.jpg]]
  
 
'''Figure 1:''' Digestion result of boiling prep plasmids.
 
'''Figure 1:''' Digestion result of boiling prep plasmids.

Revision as of 11:56, 2 October 2013

GFP nickel regulated 2

This is a nickel sensitive system driven by the promoter pexbB (BBa_K1151004) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.

Boiling prep and digestion with restriction enzymes

                         EPEP1.jpg

Figure 1: Digestion result of boiling prep plasmids.

PCR with VF2 and VR2 primers

                              PCR34.jpg

Figure 2: PCR results.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830