Difference between revisions of "Part:BBa K1151010"
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===Boiling prep and digestion with restriction enzymes=== | ===Boiling prep and digestion with restriction enzymes=== | ||
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'''Figure 1:''' Digestion result of boiling prep plasmids. | '''Figure 1:''' Digestion result of boiling prep plasmids. | ||
Revision as of 11:48, 2 October 2013
GFP nickel regulated 2
This is a nickel sensitive system driven by the promoter pexbB (BBa_K1151004) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.
Boiling prep and digestion with restriction enzymes
Figure 1: Digestion result of boiling prep plasmids.
PCR with VF2 and VR2 primers
Figure 2: PCR results.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 830