Difference between revisions of "Part:BBa K1151005"
(→‎Boiling prep and digestion with restriction enzymes)
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===Boiling prep and digestion with restriction enzymes===
 
===Boiling prep and digestion with restriction enzymes===
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Once inserted the gene coding for pUreA in a PSB1C3 plasmid (digestion and ligation), we transformed DH5a cells with the construct and then we recovered the ligated plasmid by boiling prep. We then proceeded with the digestion with EcoRI and PstI to check.
  
 
[[File:boiling1.jpg]]
 
[[File:boiling1.jpg]]
  
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Figure 1:
 
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===Usage and Biology===
 
===Usage and Biology===
  
 
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===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K1151005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1151005 SequenceAndFeatures</partinfo>

Revision as of 11:11, 2 October 2013

pUreA

This promoter derived from ureAB operon of Helicobacter pylori. It can be linked by HpNikR (BBa_K1151000) in nickel presence for promoting trascription of the downstream gene.

Boiling prep and digestion with restriction enzymes

Once inserted the gene coding for pUreA in a PSB1C3 plasmid (digestion and ligation), we transformed DH5a cells with the construct and then we recovered the ligated plasmid by boiling prep. We then proceeded with the digestion with EcoRI and PstI to check.

Boiling1.jpg

Figure 1:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]