Difference between revisions of "Part:BBa K1111012"
| Line 12: | Line 12: | ||
For us, the idea was to attach nanoparticles covered with biotin, using the strong affinity between both, but any experience implying biotin can be considered. Nanocapsules have a size comprised in a range from , which is quite big compared to a typical E:Coli cell size, we can therefore imagine attaching large proteins to these bacteria. | For us, the idea was to attach nanoparticles covered with biotin, using the strong affinity between both, but any experience implying biotin can be considered. Nanocapsules have a size comprised in a range from , which is quite big compared to a typical E:Coli cell size, we can therefore imagine attaching large proteins to these bacteria. | ||
| − | |||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 11:08, 2 October 2013
Ice Nucleation Protein fused to Streptavidin Alive
Introduction
What it is : INP sequence fused with Streptavidin Alive by gibson assembly. The INP-streptavidin construct expresses streptavidin at the outer membrane of E.Coli. INP is an outer membrane protein foud in the genome of Pseudomonas syringae. It is under the control of Lac promoter and thus is constitutively expressed in E.Coli. The expression of this plasmid was tested by transforming cells and looking for microscopy at either of a fluorescent antibody again streptavidin or a fluorescent biotin (biotin and streptavidin have a strong affinity, their Kd is 10-15 M).
Usage and Biology
For us, the idea was to attach nanoparticles covered with biotin, using the strong affinity between both, but any experience implying biotin can be considered. Nanocapsules have a size comprised in a range from , which is quite big compared to a typical E:Coli cell size, we can therefore imagine attaching large proteins to these bacteria.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1727
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1649
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1727
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1727
Illegal NgoMIV site found at 1036
Illegal AgeI site found at 1691
Illegal AgeI site found at 1742 - 1000COMPATIBLE WITH RFC[1000]