Difference between revisions of "Part:BBa K1111013:Experience"
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===Applications of BBa_K1111013=== | ===Applications of BBa_K1111013=== | ||
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| + | ===Sequencing=== | ||
| + | We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. | ||
| + | The sequencing results are shown here : | ||
| + | |||
| + | ===Microscopy=== | ||
| + | Also, we transformed cells and used them to do microscopy. Two experiments were done, one with a fluorescent antibody against streptavidin and the other one with a fluorescent biotin. We did both in case the antibody would be too big, since biotin is a small molecule best suit to cross glycocalix. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 10:45, 2 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1111013
Sequencing
We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. The sequencing results are shown here :
Microscopy
Also, we transformed cells and used them to do microscopy. Two experiments were done, one with a fluorescent antibody against streptavidin and the other one with a fluorescent biotin. We did both in case the antibody would be too big, since biotin is a small molecule best suit to cross glycocalix.
User Reviews
UNIQbbdab77a9dd2b68c-partinfo-00000000-QINU UNIQbbdab77a9dd2b68c-partinfo-00000001-QINU