Difference between revisions of "Part:BBa K1150026"
| Line 25: | Line 25: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Fusion protein of [https://parts.igem.org/Part:BBa_K1150004|PhyB] and [https://parts.igem.org/Part:BBa_K1150001|VP16]. | Fusion protein of [https://parts.igem.org/Part:BBa_K1150004|PhyB] and [https://parts.igem.org/Part:BBa_K1150001|VP16]. | ||
| − | This fusion protein PhyB-VP16 is an interaction partner of [https://parts.igem.org/Part:BBa_K1150025|dCas9-PIF]. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. When the system is exposed to red light (660 nm), the Phytochrome B receptor binds to the Phytochrome interaction actor (PIF). So it recruits the [https://parts.igem.org/Part:BBa_K1150025|dCas9-PIF] protein. | + | This fusion protein PhyB-VP16 is an interaction partner of [https://parts.igem.org/Part:BBa_K1150025|dCas9-PIF]. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. When the system is exposed to red light (660 nm), the Phytochrome B receptor binds to the Phytochrome interaction actor (PIF). So it recruits the [https://parts.igem.org/Part:BBa_K1150025|dCas9-PIF] protein. |
| − | The PhyB-PIF binding can be abolished by illumination with far-red light (740nm.) | + | The PhyB-PIF binding can be abolished by illumination with far-red light (740nm.) This system enables activation of gene expression induced by red light. |
| − | This system enables activation of gene expression induced by red light. | + | |
===References=== | ===References=== | ||
Revision as of 09:47, 2 October 2013
uniCAS Red Light Switch Part II - Activator
| uniCAS red Light Switch Part II - Activator | |
|---|---|
| Function | Activation domain of red light
induced gene expression control |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Usage and Biology
Fusion protein of [1] and [2]. This fusion protein PhyB-VP16 is an interaction partner of [3]. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. When the system is exposed to red light (660 nm), the Phytochrome B receptor binds to the Phytochrome interaction actor (PIF). So it recruits the [4] protein. The PhyB-PIF binding can be abolished by illumination with far-red light (740nm.) This system enables activation of gene expression induced by red light.
References
Müller, K.; Engesser, R.; Metzger, S.; Schulz, S.; Kampf, M. M.; Busacker, M. et al. (Nucleid Acid Research, 2013): A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
Illegal BglII site found at 1076
Illegal BamHI site found at 1158
Illegal XhoI site found at 1109
Illegal XhoI site found at 1128 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1325