Difference between revisions of "Part:BBa K1150026"
Line 23: Line 23:
 
|}
 
|}
  
 +
===Usage and Biology===
 
Fusion protein of [https://parts.igem.org/Part:BBa_K1150004|PhyB] and [https://parts.igem.org/Part:BBa_K1150001|VP16].  
 
Fusion protein of [https://parts.igem.org/Part:BBa_K1150004|PhyB] and [https://parts.igem.org/Part:BBa_K1150001|VP16].  
 
This fusion protein PhyB-VP16 is an interaction partner of [https://parts.igem.org/Part:BBa_K1150025|dCas9-PIF]. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. When the system is exposed to red light (660 nm), the Phytochrome B receptor binds to the Phytochrome interaction actor (PIF). So it recruits the [https://parts.igem.org/Part:BBa_K1150025|dCas9-PIF] protein. \\
 
This fusion protein PhyB-VP16 is an interaction partner of [https://parts.igem.org/Part:BBa_K1150025|dCas9-PIF]. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. When the system is exposed to red light (660 nm), the Phytochrome B receptor binds to the Phytochrome interaction actor (PIF). So it recruits the [https://parts.igem.org/Part:BBa_K1150025|dCas9-PIF] protein. \\
Line 28: Line 29:
 
This system enables activation of gene expression induced by red light.
 
This system enables activation of gene expression induced by red light.
  
−
===Usage and Biology===
+
===References===
 +
Müller, K.; Engesser, R.; Metzger, S.; Schulz, S.; Kampf, M. M.; Busacker, M. et al. (Nucleid Acid Research, 2013): A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells.
  
 
<!-- -->
 
<!-- -->

Revision as of 09:39, 2 October 2013

uniCAS Red Light Switch Part II - Activator


uniCAS red Light Switch Part II - Activator
Function Activation domain of red light

induced gene expression control

Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Usage and Biology

Fusion protein of [1] and [2]. This fusion protein PhyB-VP16 is an interaction partner of [3]. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. When the system is exposed to red light (660 nm), the Phytochrome B receptor binds to the Phytochrome interaction actor (PIF). So it recruits the [4] protein. \\ The PhyB-PIF binding can be abolished by illumination with far-red light (740nm.)\\ This system enables activation of gene expression induced by red light.

References

Müller, K.; Engesser, R.; Metzger, S.; Schulz, S.; Kampf, M. M.; Busacker, M. et al. (Nucleid Acid Research, 2013): A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 1076
    Illegal BamHI site found at 1158
    Illegal XhoI site found at 1109
    Illegal XhoI site found at 1128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1325