Difference between revisions of "Part:BBa K1150026"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1150026 short</partinfo>
 
<partinfo>BBa_K1150026 short</partinfo>
  
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..
 
  
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<!-- Add more about the biology of this part here
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|PhyB
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|-
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|'''Function'''
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|Activation domain of red light
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induced gene expression control
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
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|}
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Fusion protein of [https://parts.igem.org/Part:BBa_K1150004|PhyB] and [https://parts.igem.org/Part:BBa_K1150001|VP16].
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This fusion protein PhyB-VP16 is an interaction partner of dCas9-UVR8. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. If the system is exposed to UVB light (311 nm), the UVR8 receptor changes its configuration and recruits this COP1-VP16 protein. This activates gene expression induced by UVB light
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 09:15, 2 October 2013

uniCAS Red Light Switch Part II - Activator


PhyB
Function Activation domain of red light

induced gene expression control

Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Fusion protein of [1] and [2]. This fusion protein PhyB-VP16 is an interaction partner of dCas9-UVR8. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. If the system is exposed to UVB light (311 nm), the UVR8 receptor changes its configuration and recruits this COP1-VP16 protein. This activates gene expression induced by UVB light

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 1076
    Illegal BamHI site found at 1158
    Illegal XhoI site found at 1109
    Illegal XhoI site found at 1128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1325