Difference between revisions of "Part:BBa K1141002:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K1141002 short</partinfo>
 
<partinfo>BBa_K1141002 short</partinfo>
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===Design Notes===
 
===Design Notes===
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There was an Ecor1 restriction site. We removed this site in order to made this biobrick compatible to the 3A assembly.
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In the original sequence coming from the pQE30 plasmid (see part BBa_K1141001) there was an Ecor1 restriction site between the promoter and the RBS. We removed this site in order to made this biobrick compatible with RFC[10], and 3A assembly.
  
  
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===Source===
 
===Source===
  
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KillerRed came from addgene plasmid pBabe-KR-CENPA
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The KillerRed coding sequence originally came from the ADDGENE plasmid pBabe-KR-CENPA
  
 
===References===
 
===References===

Revision as of 21:20, 1 October 2013

Plac-RBS-KillerRed (IPTG-inducible)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 133
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 289
    Illegal BsaI.rc site found at 580


Design Notes

In the original sequence coming from the pQE30 plasmid (see part BBa_K1141001) there was an Ecor1 restriction site between the promoter and the RBS. We removed this site in order to made this biobrick compatible with RFC[10], and 3A assembly.


Source

The KillerRed coding sequence originally came from the ADDGENE plasmid pBabe-KR-CENPA

References