Difference between revisions of "Part:BBa K1141002"

 
(Corrected main description and added info for biological use of the part)
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<partinfo>BBa_K1141002 short</partinfo>
 
<partinfo>BBa_K1141002 short</partinfo>
  
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KillerRed is a red fluorescent protein wich is able to produce ROS under an illumination. We construct a biobrick where the production of KillerRed is under the control of pLAC promoter. So the production of KillerRed is IPTG dependent. We used this part to trigger cell death in response to light illumination for controlling cell density.
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KillerRed is a red fluorescent protein which produces ROS under green illumination (roughly 500-600 nm, absorption peak at 535 nm). This biobrick allows production of KillerRed under the control of the PLAC promoter (In this construction, actually a T5 phage promoter preceded by two lac operators for strong repression in presence of LacI). So the production of KillerRed is IPTG dependent. We used this part to trigger cell death in response to light illumination for controlling cell density in the Talk'E.coli project of Grenoble-EMSE-LSU 2013.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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This part is a protein generator with integrated promoter, in a single piece. The KillerRed protein coding sequence can be obtained by PCR to be placed in other BioBrick constructions.
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The IPTG-inducible PLAC promoter allows precise control of protein expression in a culture. It is strongly advised to use a strain with strong repression of PLAC, such as QIAGEN's M15 cells with the pREP4 plasmid (this plasmid can be transfected into any wild-type strain in order to confer strong PLAC repression).
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 21:16, 1 October 2013

Plac-RBS-KillerRed (IPTG-inducible)

KillerRed is a red fluorescent protein which produces ROS under green illumination (roughly 500-600 nm, absorption peak at 535 nm). This biobrick allows production of KillerRed under the control of the PLAC promoter (In this construction, actually a T5 phage promoter preceded by two lac operators for strong repression in presence of LacI). So the production of KillerRed is IPTG dependent. We used this part to trigger cell death in response to light illumination for controlling cell density in the Talk'E.coli project of Grenoble-EMSE-LSU 2013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 133
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 289
    Illegal BsaI.rc site found at 580