Difference between revisions of "Part:BBa K1075025"

Revision as of 20:48, 1 October 2013

pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT pLac2-RBS34-mCherry-ssrA-TT

Usage and Biology

The ssrA tag is a sequence, which allows proteolytic enzymes to degrade them. It relates to the protease ClpXP complex in E.coli and it also allows adaptor proteins such as sspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutation ssrA (DAS+4) prevents a direct binding to the protease and ensures the dependance of sspB. The tagged protein in this case is mCherry, a red fluorescing protein. Degradation can be measured by the decrease of light intensity. Prefixed is RBS, the binding site for a ribosome and the pLac promoter. It also includes a double terminator 'TT', which interrupts the translation. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1717
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1075025 short</partinfo>
+pLac2-RBS34-mCherry-ssrA-TT
-a
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The ssrA tag is a sequence, which allows proteolytic enzymes to degrade them. It relates to the protease ClpXP  complex in E.coli and it also allows adaptor proteins such as sspB binding and delivering substrates to the proteases in order to make the process more efficient.
+The mutation ssrA (DAS+4) prevents a direct binding to the protease and ensures the dependance of sspB.
+The tagged protein in this case is mCherry, a red fluorescing protein. Degradation can be measured by the decrease of light intensity.
+Prefixed is RBS, the binding site for a ribosome and the pLac promoter. It also includes a double terminator 'TT', which interrupts the translation.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>