Difference between revisions of "Part:BBa K1104208"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1104208 short</partinfo>
 
<partinfo>BBa_K1104208 short</partinfo>
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[[File: NYMU_DsbGp.png|frame|center|'''DsbGp''']]
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We improve the function of a BioBrick Part: AhpC promoter([https://parts.igem.org/Part:BBa_K362001 K362001])designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team] into four versions.On PartRegistry, the complex part(according to [http://ecocyc.org/ECOLI/new-image?object=EG11384 Ecocyc]) composition contains hybrid promoters(AhpC1, AhpC2, and DsbG promoter), shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG.
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There is only DsbGp promoter in this part. We designed the new part and test the promoter separately.
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[[File: NYMU_Ax.png|thumb|600px|center|'''Improvement of ahpC promoter([https://parts.igem.org/Part:BBa_K362001 Part:K362001])''']]
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===Usage and Biology===
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We designed circuit fighting against ''Nosema ceranae''. After Nosema ceranae infected midgut cells of bees, and Bee. coli should sense the pathogen first before the following circuit(fighting against Nosema ceranae)is triggered, and substance such as [https://parts.igem.org/Part:BBa_K1104300 Defensin(Part:BBa_K1104300)], [https://parts.igem.org/Part:BBa_K1104301  Abaesin(Part:BBa_K1104301)] (more details on [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Killing Killing Nosema] page) in the following circuit will express.
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To enhance the strength , we added a device (more details on [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema] page).
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[[File: NYMU_O12.png|frame|center|'''Strenthening device''']]
  
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We improve the function of a BioBrick Part: AhpC promoter(K362001)designed by 2010 KIT-Tokyo team into four versions.On PartRegistry, the complex part(according to Ecocyc) composition contains hybrid promoters(AhpC1, AhpC2, and DsbG promoter), shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG. There is only AhpC1 promoter in this part. We designed the new part and test the promoter alone.
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===Related Parts===
  
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*ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)]
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**AhpCp1000([https://parts.igem.org/Part:BBa_K1104203 Part:BBa_K1104203])
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***[https://parts.igem.org/Part:BBa_K1104242 Part:BBa_K1104243]: AhpCp1000+[https://parts.igem.org/Part:BBa_E0840 E0840]
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**AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204])
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***[https://parts.igem.org/Part:BBa_K1104244 Part:BBa_K1104244]: AhpCp2D1+[https://parts.igem.org/Part:BBa_E0840 E0840]
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**AhpCp2([https://parts.igem.org/Part:BBa_K1104205 Part:BBa_K1104205])
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***[https://parts.igem.org/Part:BBa_K1104245 Part:BBa_K1104245]: AhpCp2+[https://parts.igem.org/Part:BBa_E0840 E0840]
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**AhpCpD1([https://parts.igem.org/Part:BBa_K1104206 Part:BBa_K1104206])
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***[https://parts.igem.org/Part:BBa_K1104246 Part:BBa_K1104246]: AhpCpD1+[https://parts.igem.org/Part:BBa_E0840 E0840]
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**AhpCp1([https://parts.igem.org/Part:BBa_K1104207 Part:BBa_K1104207])
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***[https://parts.igem.org/Part:BBa_K1104247 Part:BBa_K1104247]: AhpCp1+[https://parts.igem.org/Part:BBa_E0840 E0840]
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**DsbGp([https://parts.igem.org/Part:BBa_K1104208 Part:BBa_K1104208])
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***[https://parts.igem.org/Part:BBa_K1104248 Part:BBa_K1104248]: DsbGp+[https://parts.igem.org/Part:BBa_E0840 E0840]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 20:38, 1 October 2013

DsbGp

DsbGp

We improve the function of a BioBrick Part: AhpC promoter(K362001)designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team] into four versions.On PartRegistry, the complex part(according to [http://ecocyc.org/ECOLI/new-image?object=EG11384 Ecocyc]) composition contains hybrid promoters(AhpC1, AhpC2, and DsbG promoter), shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG. There is only DsbGp promoter in this part. We designed the new part and test the promoter separately.

Improvement of ahpC promoter(Part:K362001)

Usage and Biology

We designed circuit fighting against Nosema ceranae. After Nosema ceranae infected midgut cells of bees, and Bee. coli should sense the pathogen first before the following circuit(fighting against Nosema ceranae)is triggered, and substance such as Defensin(Part:BBa_K1104300), Abaesin(Part:BBa_K1104301) (more details on [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Killing Killing Nosema] page) in the following circuit will express.

To enhance the strength , we added a device (more details on [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema] page).

Strenthening device

Related Parts

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]