Difference between revisions of "Part:BBa K1104206"
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:OxyR binding to the ahpC-proximal site leads to the induction of both dsbG and ahpC transcripts, while OxyR binding to the dsbG-proximal site leads to the induction of a second ahpC transcript. This transcript of ahpC and the transcript of dsbG overlap by over 100 nucleotides. | :OxyR binding to the ahpC-proximal site leads to the induction of both dsbG and ahpC transcripts, while OxyR binding to the dsbG-proximal site leads to the induction of a second ahpC transcript. This transcript of ahpC and the transcript of dsbG overlap by over 100 nucleotides. | ||
:The intergenic region between dsbG and ahpC carries two binding sites for OxyR, a dsbG-proximal site, located 54 bp upstream of the dsbG start codon; an ahpC-proximal site, located 290 bp upstream of the dsbG start codon. | :The intergenic region between dsbG and ahpC carries two binding sites for OxyR, a dsbG-proximal site, located 54 bp upstream of the dsbG start codon; an ahpC-proximal site, located 290 bp upstream of the dsbG start codon. | ||
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| + | ===Usage and Biology=== | ||
| + | There are two TFBSs between AhpCp1 and reverse DsbGp. OxyR binding to the ahpC-proximal site leads to the induction of both dsbG and ahpC transcripts. | ||
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| + | Once ROS(Reactive Oxygen Species) oxidizes OxyR, making OxyR interact with the TFBS between AhpCp1 and DsbGp, dual-directional transcription will occur. It can be take advantages of by certain special circuit design. | ||
===Related Parts=== | ===Related Parts=== | ||
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Revision as of 20:35, 1 October 2013
AhpCpD1
AhpCpD1
- We improve the function of a BioBrick Part: ahpC promoter(K362001)designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team] into four versions.On PartRegistry, the complex part(according to [http://ecocyc.org/ECOLI/new-image?object=EG11384 Ecocyc]) composition contains hybrid promoters, shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG.
Improvement of ahpC promoter(Part:K362001)
- OxyR binding to the ahpC-proximal site leads to the induction of both dsbG and ahpC transcripts, while OxyR binding to the dsbG-proximal site leads to the induction of a second ahpC transcript. This transcript of ahpC and the transcript of dsbG overlap by over 100 nucleotides.
- The intergenic region between dsbG and ahpC carries two binding sites for OxyR, a dsbG-proximal site, located 54 bp upstream of the dsbG start codon; an ahpC-proximal site, located 290 bp upstream of the dsbG start codon.
Usage and Biology
There are two TFBSs between AhpCp1 and reverse DsbGp. OxyR binding to the ahpC-proximal site leads to the induction of both dsbG and ahpC transcripts.
Once ROS(Reactive Oxygen Species) oxidizes OxyR, making OxyR interact with the TFBS between AhpCp1 and DsbGp, dual-directional transcription will occur. It can be take advantages of by certain special circuit design.
Related Parts
- ahpC(Part:BBa_K362001)
- AhpCp1000(Part:BBa_K1104203)
- Part:BBa_K1104243: AhpCp1000+E0840
- AhpCp2D1(Part:BBa_K1104204)
- Part:BBa_K1104244: AhpCp2D1+E0840
- AhpCp2(Part:BBa_K1104205)
- Part:BBa_K1104245: AhpCp2+E0840
- AhpCpD1(Part:BBa_K1104206)
- Part:BBa_K1104246: AhpCpD1+E0840
- AhpCp1(Part:BBa_K1104207)
- Part:BBa_K1104247: AhpCp1+E0840
- DsbGp(Part:BBa_K1104208)
- Part:BBa_K1104248: DsbGp+E0840
- AhpCp1000(Part:BBa_K1104203)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]