Difference between revisions of "Part:BBa K1075020"

 
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fused to the C terminus of a protein it triggers degradation upon induction of sspB function.
 
fused to the C terminus of a protein it triggers degradation upon induction of sspB function.
  
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===Usage and Biology===
 
===Usage and Biology===
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The ssrA tag is a sequence, which allows proteolytic enzymes to degrade them. It relates to the protease ClpXP  complex in E.coli and it also allows adaptor proteins such as sspB binding and delivering substrates to the proteases in order to make the process more efficient.
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The mutation ssrA (DAS+4) prevents a direct binding to the protease and ensures the dependance of sspB.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1075020 SequenceAndFeatures</partinfo>
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Revision as of 20:30, 1 October 2013

E. coli ssrA(DAS+4)

fused to the C terminus of a protein it triggers degradation upon induction of sspB function.


Usage and Biology

The ssrA tag is a sequence, which allows proteolytic enzymes to degrade them. It relates to the protease ClpXP complex in E.coli and it also allows adaptor proteins such as sspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutation ssrA (DAS+4) prevents a direct binding to the protease and ensures the dependance of sspB.