Difference between revisions of "Part:BBa K1189000"
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<img src="https://static.igem.org/mediawiki/parts/4/4d/YYC2013_TALE_Construct_His_TALE_A_White_Background.jpg"> | <img src="https://static.igem.org/mediawiki/parts/4/4d/YYC2013_TALE_Construct_His_TALE_A_White_Background.jpg"> | ||
</figure> | </figure> | ||
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</html> | </html> | ||
+ | <html><figcaption>Fig 1: Shows the repeat variable domain(RVD) of TALEs. These RVDs are very specific and the code of 2 amino acids that bind to each nucleotide has been solved making TALEs very easy to engineer and very specific to their targets</figcaption></html> | ||
[[File:Calgary2013-TALE-RVD.png]] | [[File:Calgary2013-TALE-RVD.png]] |
Revision as of 20:08, 1 October 2013
TALE-A with a his tag under a lacI promoter
This part was built to allow for the extraction of TALA proteins with the his-tags. This part was also built with J04500, an IPTG inducible promoter with RBS. TALA proteins can specifically bind to DNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2654
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]