Difference between revisions of "Part:BBa K1189001"
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[[File:Calgary2013-TALE-RVD.png]]
 
[[File:Calgary2013-TALE-RVD.png]]
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<figcaption>Fig 1: Shows the repeat variable domain(RVD) of TALEs. These RVDs are very specific and the code of 2 amino acids that bind to each nucleotide has been solved making TALEs very easy to engineer and very specific to their targets</figcaption>
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<html><figcaption>Fig 1: Shows the repeat variable domain(RVD) of TALEs. These RVDs are very specific and the code of 2 amino acids that bind to each nucleotide has been solved making TALEs very easy to engineer and very specific to their targets</figcaption></html>
  
 
[[File:Ucalgary_TALE-B_kozak!.png|200px]]
 
[[File:Ucalgary_TALE-B_kozak!.png|200px]]
 
<html>
 
<html>
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<figcaption>Fig 2: Western blot showing the expression levels of TALE-B with and without Kozak sequences. We show that in the presence of kozak sequence TALE-B is not expressed however once we remove the kozak sequence TALE-B is expressed.</figcaption>
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<figcaption>Fig 2: Western blot showing the expression levels of TALE-B with and without Kozak sequences. We show that in the presence of kozak sequence TALE-B is not expressed however once we remove the kozak sequence TALE-B is expressed.</figcaption></html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:05, 1 October 2013

TALE-B with a his 6 tag under a lacI promoter

This part was built to allow for the extraction of TALB proteins with the his-tags. This part was also built with J04500, an IPTG inducible promoter with RBS. TALB proteins can specifically bind to DNA.

Calgary2013-TALE-RVD.png

Fig 1: Shows the repeat variable domain(RVD) of TALEs. These RVDs are very specific and the code of 2 amino acids that bind to each nucleotide has been solved making TALEs very easy to engineer and very specific to their targets

Ucalgary TALE-B kozak!.png

Fig 2: Western blot showing the expression levels of TALE-B with and without Kozak sequences. We show that in the presence of kozak sequence TALE-B is not expressed however once we remove the kozak sequence TALE-B is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]