Difference between revisions of "Part:BBa K1045017"

Latest revision as of 19:39, 1 October 2013

DarR reporter system

This composite part encodes a reporter system to monitor the repression mediated by DarR. DarR is a transcriptional repressor that binds to the DarR operator sequence blocking transcription of the downstream gene. These effects are enhanced by c-di-AMP. In part BBa_K1045017, the DarR operator is placed in between a strong promoter BBa_J23110 and a GFP generator BBa_E0240. DarR itself is part of an inverted expression unit with a weak promoter BBa_J23117. Transcription of the DarR mRNA might be terminated by an inverse terminator BBa_K1045009. This system was designed to screen for compounds similar to c-di-AMP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 806
Illegal NheI site found at 829
Illegal NheI site found at 909
Illegal NheI site found at 932
Illegal NotI site found at 718
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 731
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 860
Illegal AgeI site found at 168
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1630

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1045017 short</partinfo>
-This composite part encodes a reporter system to monitor the repression mediated by DarR. DarR is a transcriptional repressor that binds to the DarR operator sequence blocking transcription of the downstream gene. These effects are enhanced by c-di-AMP. In part [[Part:BBa_K1045017|BBa_K1045017]], the DarR operator is placed in between a strong promoter [[Part:BBa_J23110|BBa_J23110]] and a GFP generator [[Part:BBa_E0240|BBa_E0240]]. DarR itself is part of an inverted expression unit with a weak promoter [[Part:BBa_J23117|BBa_J23117]]. Transcription of the DarR mRNA might be terminated by an inverse terminator [[Part:BBa_K104509|BBa_K104509]]. This system was designed to screen for compounds similar to c-di-AMP.
+This composite part encodes a reporter system to monitor the repression mediated by DarR. DarR is a transcriptional repressor that binds to the DarR operator sequence blocking transcription of the downstream gene. These effects are enhanced by c-di-AMP. In part [[Part:BBa_K1045017|BBa_K1045017]], the DarR operator is placed in between a strong promoter [[Part:BBa_J23110|BBa_J23110]] and a GFP generator [[Part:BBa_E0240|BBa_E0240]]. DarR itself is part of an inverted expression unit with a weak promoter [[Part:BBa_J23117|BBa_J23117]]. Transcription of the DarR mRNA might be terminated by an inverse terminator [[Part:BBa_K1045009|BBa_K1045009]]. This system was designed to screen for compounds similar to c-di-AMP.
 <!-- Add more about the biology of this part here