Difference between revisions of "Part:BBa K1104200"

(Usage and Biology)
(Introduction)
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===Introduction===
 
===Introduction===
 
OxyR is Escherichia coli transcription factor that senses
 
OxyR is Escherichia coli transcription factor that senses
H<sub>2</sub>O<sub>2</sub> to activate transcription, and is activated through the formation of an intramolecular disulfide bond.
+
H<sub>2</sub>O<sub>2</sub> to activate transcription, and is activated through the formation of an intramolecular disulfide bond. The OxyR gene, a positive regulatory gene for the oxidative response, then controls proteins which are induced by treatment with H<sub>2</sub>O<sub>2</sub>.  
  
 
[[File: NYMU_NYMU_OxyRcod.png|frame|center|'''OxyR([https://parts.igem.org/BBa_K1104200 BBa_K1104200])''']]
 
[[File: NYMU_NYMU_OxyRcod.png|frame|center|'''OxyR([https://parts.igem.org/BBa_K1104200 BBa_K1104200])''']]

Revision as of 19:12, 1 October 2013

OxyR

Introduction

OxyR is Escherichia coli transcription factor that senses H2O2 to activate transcription, and is activated through the formation of an intramolecular disulfide bond. The OxyR gene, a positive regulatory gene for the oxidative response, then controls proteins which are induced by treatment with H2O2.

Literature Study of OxyR

Illustration of OxyR Activation

OxyR, a positive regulator of hydrogen peroxide-inducible genes in E. coli. The OxyR transcription factor regulates the H2O2 response of E. coli. H2O2 oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.

Structure of the OxyR Regulatory Domain
(A)Reduced Form: Inactive
(B)Oxidized Form: Activated by ROS
Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.


Usage and Biology

After Nosema ceranae enter bees' midgut, the natural immune reaction will lead to ROS released by epithelia of bees' midgut. In our project: Bee. coli, we use OxyR-induced promoter as the sensor of Bee. coli to sense Nosema ceranae. We select several promoters that can be induced by OxyR(Part:BBa_K1104200).

There are promoters that can be activated by OxyR: TrxCp(Part:BBa_K1104201), HemHp(Part:BBa_K1104202), ahpC(Part:BBa_K362001), sufA(Part:BBa_K362005), and dps(Part:BBa_K362002), yiaA(Part:BBa_K362006)

The OxyR-induced promoters:

  • TrxCp(Part:BBa_K1104201) and HemHp(Part:BBa_K1104202) are parts created by our team [http://2013.igem.org/Team:NYMU-Taipei/Modeling/MainParts NYMU_Taipei].(Come to the [http://2013.igem.org/Team:NYMU-Taipei/Experiment/Wet_Lab Wet Lab] page to see our part list.)
  • sufA(Part:BBa_K362005), designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team], is successfully characterized again by us.

In order to enhance the strength and sensitivity of ROS-sensing promoters, we make E. coli continuously produce inactivated OxyR transcription factors, so that ROS can affect promoters more easily.

We designed a device that is composed of a sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) plus reporter (Part:BBa_E0840). Device1 in order to enhance the effect of ROS on E. coli is added ahead: a constitutive promoter(Part:BBa_J23102), and OxyR(Part:BBa_K1104200).

Related Parts

  1. TrxCp(Part:BBa_K1104201)
  2. Part:BBa_K1104241: TrxCp+E0840
  3. HemHp(Part:BBa_K1104202)
  4. Part:BBa_K1104242: HemHp+E0840
  5. sufA(Part:BBa_K362005)
  6. ahpC(Part:BBa_K362001)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]