Difference between revisions of "Part:BBa K1033000:Design"

 
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<partinfo>BBa_K1033000 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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Earlier in iGEM eucaryot TAL has been used, however we used bacterial TAL for better compatibility with E.coli.
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Earlier in iGEM eukaryot TAL has been used, however we used bacterial TAL for better compatibility with bacteria.  
  
  

Revision as of 18:56, 1 October 2013

Tyrosine ammonia-lyase (TAL) with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 45
    Illegal NgoMIV site found at 877
    Illegal AgeI site found at 140
    Illegal AgeI site found at 306
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1335


Design Notes

Earlier in iGEM eukaryot TAL has been used, however we used bacterial TAL for better compatibility with bacteria.


Source

Rhodobacter sphaeroides

References