Difference between revisions of "Part:BBa K1111009"

Revision as of 18:40, 1 October 2013

Gelatinase under pBAD/araC arabinose inducible promoter

This construct is composed of gelatinase E's (gelE) coding sequence(CDS)inserted between the pBAD/araC promoter and superfolder GFP of part BBa_I746908 from Cambridge 2007. A linker is also present to join gelE and GFP correctly and to allow proper folding. Upon arabinose addition in the culture medium of bacteria transformed with this plasmid, gelE-GFP will be expressed and secreted

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 3101
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
Illegal PstI site found at 3101
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2634
Illegal BglII site found at 2685
Illegal BamHI site found at 1144
Illegal BamHI site found at 3028
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 3101
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 3101
Illegal NgoMIV site found at 2330
Illegal AgeI site found at 979
Illegal AgeI site found at 2703
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1517
Illegal BsaI.rc site found at 2234
Illegal SapI site found at 961
Illegal SapI.rc site found at 3169

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1111009 short</partinfo>
-This construct expresses gelatinase upon addition of arabinose. Once expressed if can degrade gelatine.
+This construct is composed of gelatinase E's (gelE) coding sequence(CDS)inserted between the pBAD/araC promoter and superfolder GFP of part BBa_I746908 from Cambridge 2007. A linker is also present to join gelE and GFP correctly and to allow proper folding.
+Upon arabinose addition in the culture medium of bacteria transformed with this plasmid, gelE-GFP will be expressed and secreted
 <!-- Add more about the biology of this part here