Difference between revisions of "Part:BBa K1104200"

Revision as of 18:11, 1 October 2013

OxyR

Introduction

OxyR is Escherichia coli transcription factor that senses H₂O₂ to activate transcription, and is activated through the formation of an intramolecular disulfide bond.

OxyR(BBa_K1104200)

Literature Study of OxyR

Illustration of OxyR Activation

OxyR, a positive regulator of hydrogen peroxide-inducible genes in E. coli. The OxyR transcription factor regulates the H₂O₂ response of E. coli. H₂O₂ oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.

Structure of the OxyR Regulatory Domain
(A)Reduced Form: Inactive
(B)Oxidized Form: Activated by ROS

_{Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.}

Usage and Biology

After Nosema ceranae enter bees' midgut, the natural immune reaction will lead to ROS released by epithelia of bees' midgut. In our project: Bee. coli, we use OxyR-induced promoter as the sensor of Bee. coli to sense Nosema ceranae. We select several promoters that can be induced by OxyR(Part:BBa_K1104200).

There are promoters that can be activated by OxyR: TrxCp(Part:BBa_K1104201), HemHp(Part:BBa_K1104202), ahpC(Part:BBa_K362001), sufA(Part:BBa_K362005).

The OxyR-induced promoters:

TrxCp(Part:BBa_K1104201) and HemHp(Part:BBa_K1104202) are parts created by our team [http://2013.igem.org/Team:NYMU-Taipei/Modeling/MainParts NYMU_Taipei].(Come to the [http://2013.igem.org/Team:NYMU-Taipei/Experiment/Wet_Lab Wet Lab] page to see our part list.)

sufA(Part:BBa_K362005), designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team], is successfully characterized again by us.

ahpC(Part:BBa_K362001) is improved its complex composition and Assembly Compatibility by us. We designed ahpC(Part:BBa_K362001) improvement into 6 versions:
- AhpCp1000(Part:BBa_K1104203)
- AhpCp2D1(Part:BBa_K1104204)
- AhpCp2(Part:BBa_K1104205)
- AhpCpD1(Part:BBa_K1104206)
- AhpCp1(Part:BBa_K1104207)
- DsbGp(Part:BBa_K1104208)

Related Parts

TrxCp(Part:BBa_K1104201)

Part:BBa_K1104241: TrxCp+E0840

HemHp(Part:BBa_K1104202)

Part:BBa_K1104242: HemHp+E0840

sufA(Part:BBa_K362005)
ahpC(Part:BBa_K362001)

- AhpCp1000(Part:BBa_K1104203)
  - Part:BBa_K1104243: AhpCp1000+E0840
- AhpCp2D1(Part:BBa_K1104204)
  - Part:BBa_K1104244: AhpCp2D1+E0840
- AhpCp2(Part:BBa_K1104205)
  - Part:BBa_K1104245: AhpCp2+E0840
- AhpCpD1(Part:BBa_K1104206)
  - Part:BBa_K1104246: AhpCpD1+E0840
- AhpCp1(Part:BBa_K1104207)
  - Part:BBa_K1104247: AhpCp1+E0840
- DsbGp(Part:BBa_K1104208)
  - Part:BBa_K1104248: DsbGp+E0840

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 17: / Line 17: @@
 ==Usage and Biology==
+After ''Nosema ceranae'' enter bees' midgut, the natural immune reaction will lead to ROS released by epithelia of bees' midgut. In our project: ''Bee. coli'', we use OxyR-induced promoter as the sensor of ''Bee. coli'' to sense ''Nosema ceranae''. We select several promoters that can be induced by OxyR([https://parts.igem.org/Part:BBa_K1104200 Part:BBa_K1104200]).
 There are promoters that can be activated by OxyR: TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201]), HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202]), ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)], sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005]).
@@ Line 34: / Line 37: @@
 ===Related Parts===
 #TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201])
+*[https://parts.igem.org/Part:BBa_K1104241 Part:BBa_K1104241]: TrxCp+[https://parts.igem.org/Part:BBa_E0840 E0840]
 #HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202])
+*[https://parts.igem.org/Part:BBa_K1104242 Part:BBa_K1104242]: HemHp+[https://parts.igem.org/Part:BBa_E0840 E0840]
 #sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005])
 #ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)]
 **AhpCp1000([https://parts.igem.org/Part:BBa_K1104203 Part:BBa_K1104203])
+***[https://parts.igem.org/Part:BBa_K1104242 Part:BBa_K1104243]: AhpCp1000+[https://parts.igem.org/Part:BBa_E0840 E0840]
 **AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204])
+***[https://parts.igem.org/Part:BBa_K1104244 Part:BBa_K1104244]: AhpCp2D1+[https://parts.igem.org/Part:BBa_E0840 E0840]
 **AhpCp2([https://parts.igem.org/Part:BBa_K1104205 Part:BBa_K1104205])
+***[https://parts.igem.org/Part:BBa_K1104245 Part:BBa_K1104245]: AhpCp2+[https://parts.igem.org/Part:BBa_E0840 E0840]
 **AhpCpD1([https://parts.igem.org/Part:BBa_K1104206 Part:BBa_K1104206])
+***[https://parts.igem.org/Part:BBa_K1104246 Part:BBa_K1104246]: AhpCpD1+[https://parts.igem.org/Part:BBa_E0840 E0840]
 **AhpCp1([https://parts.igem.org/Part:BBa_K1104207 Part:BBa_K1104207])
+***[https://parts.igem.org/Part:BBa_K1104247 Part:BBa_K1104247]: AhpCp1+[https://parts.igem.org/Part:BBa_E0840 E0840]
 **DsbGp([https://parts.igem.org/Part:BBa_K1104208 Part:BBa_K1104208])
+***[https://parts.igem.org/Part:BBa_K1104248 Part:BBa_K1104248]: DsbGp+[https://parts.igem.org/Part:BBa_E0840 E0840]
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