Difference between revisions of "Part:BBa K1104200"

Revision as of 17:39, 1 October 2013

OxyR

Introduction

The Escherichia coli OxyR transcription factor senses H₂O₂ to activate transcription, and is activated through the formation of an intramolecular disulfide bond.

There are promoters that can be activated by OxyR: TrxCp(Part:BBa_K1104201), HemHp(Part:BBa_K1104202), ahpC(Part:BBa_K362001), sufA(Part:BBa_K362005).

The OxyR-induced promoters:

TrxCp(Part:BBa_K1104201) and HemHp(Part:BBa_K1104202) are parts created by our team [http://2013.igem.org/Team:NYMU-Taipei/Modeling/MainParts NYMU_Taipei].(Come to the [http://2013.igem.org/Team:NYMU-Taipei/Experiment/Wet_Lab Wet Lab] page to see our part list.)

sufA(Part:BBa_K362005), designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team], is successfully characterized again by us.

ahpC(Part:BBa_K362001) is improved its complex composition and Assembly Compatibility by us. We designed ahpC(Part:BBa_K362001) improvement into 6 versions:
- AhpCp1000(Part:BBa_K1104203)
- AhpCp2D1(Part:BBa_K1104204)
- AhpCp2(Part:BBa_K1104205)
- AhpCpD1(Part:BBa_K1104206)
- AhpCp1(Part:BBa_K1104207)
- DsbGp(Part:BBa_K1104208)

Literature Study of OxyR

Illustration of OxyR Activation

OxyR, a positive regulator of hydrogen peroxide-inducible genes in E. coli. The OxyR transcription factor regulates the H₂O₂ response of E. coli. H₂O₂ oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.

Structure of the OxyR Regulatory Domain
(A)Reduced Form: Inactive
(B)Oxidized Form: Activated by ROS

_{Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.}

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 There are promoters that can be activated by OxyR: TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201]), HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202]), ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)], sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005]).
-The OxyR-induced promoters: TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201]) and HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202]) are parts created by our team [http://2013.igem.org/Team:NYMU-Taipei/Modeling/MainParts NYMU_Taipei].(Come to the [http://2013.igem.org/Team:NYMU-Taipei/Experiment/Wet_Lab Wet Lab] page to see our part list.)
+The OxyR-induced promoters:
+*TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201]) and HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202]) are parts created by our team [http://2013.igem.org/Team:NYMU-Taipei/Modeling/MainParts NYMU_Taipei].(Come to the [http://2013.igem.org/Team:NYMU-Taipei/Experiment/Wet_Lab Wet Lab] page to see our part list.)
-The OxyR-induced promoters: sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005]) that is
+*sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005]), designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team], is successfully characterized again by us.
+*ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)] is improved its complex composition and Assembly Compatibility by us. We designed ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)] improvement into 6 versions:
+**AhpCp1000([https://parts.igem.org/Part:BBa_K1104203 Part:BBa_K1104203])
+**AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204])
+**AhpCp2([https://parts.igem.org/Part:BBa_K1104205 Part:BBa_K1104205])
+**AhpCpD1([https://parts.igem.org/Part:BBa_K1104206 Part:BBa_K1104206])
+**AhpCp1([https://parts.igem.org/Part:BBa_K1104207 Part:BBa_K1104207])
+**DsbGp([https://parts.igem.org/Part:BBa_K1104208 Part:BBa_K1104208])
 ===Literature Study of OxyR===