Difference between revisions of "Part:BBa K1151006"
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It's a simple coding device of HpNikR, obtained as ligation product of BBa_J04500 + [[BBa_K1151000]] + BBa_B0015. The protein is synthesized when IPTG is present.
 
It's a simple coding device of HpNikR, obtained as ligation product of BBa_J04500 + [[BBa_K1151000]] + BBa_B0015. The protein is synthesized when IPTG is present.
  
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===Protein expression===
 
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The plasmid containing this part has been inserted inside of BL21 cells, and we proceeded with the classic protocol of induction with IPTG for a time of 1, 2 and 4 hours.
 
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                                              [[File:rit.jpg]]
 
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'''Figure 1:''' Induction results.
 
  
 
===Sequence and Features===
 
===Sequence and Features===

Revision as of 17:17, 1 October 2013

HpNikR coding device

It's a simple coding device of HpNikR, obtained as ligation product of BBa_J04500 + BBa_K1151000 + BBa_B0015. The protein is synthesized when IPTG is present.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]